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KMID : 0616120110420020138
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Korean Journal of Pharmacognosy
2011 Volume.42 No. 2 p.138 ~ p.143
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Contents of Poncirin and Naringin in Fruit of Poncirus Trifoliata according to Different Harvesting Times and Locations for Two Years
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Lee Sul-Lim
Seo Chang-Seob
Kim Jung-Hoon
Shin Hyeun-Kyoo
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Abstract
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A high performance liquid chromatography (HPLC) method was developed for simultaneous determination of two major flavonoid glycosides (poncirin and nanringin) in Poncirus trifoliata Raf. by different harvesting times and locations for two years. A SunFire column (4.6 mm250 mm, 5 ) was used at for the determination of poncirin and naringin. The mobile phase using gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Flow rate was 1.0 mL/min and injection volume was 10 . The chromatogram was monitored by photodiode array (PDA) detection at 280 nm for the identification of two flavonoid glycosides in P. trifoliata. The contents of the two components in P. trifoliata ranged from 0.32~13.02%.
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KEYWORD
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Poncirus trifoliata, HPLC, Poncirin, Naringin
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